Journal: Kidney international

Article Title: Expression of urokinase plasminogen activator and its receptor during acute renal allograft rejection.

doi: 10.1046/j.1523-1755.2003.00261.x

Figure Lengend Snippet: Fig. 1. Correlation between urokinase plasminogen activator receptor (uPAR) level in serum (A), in urine (B), and the serum creatinine concentration. Serum and urine levels of uPAR were determined by enzyme-linked immunosorbent assay (ELISA). The urine and serum levels of uPAR correlate with the renal function, determined by mean creatinine levels in the blood (serum R 0.68, P 0.01; urine R 0.50, P 0.01; Spearman rho test).

Article Snippet: uPAR concentrations in urine and serum were measured by ELISA according to the manufacturer using purified mouse antihuman uPAR monoclonal antibody (mAb) (4 g/mL) (R&D systems, Abingdon, UK) as coating antibody, biotinylated goat-antihuman uPAR antibody (100 ng/mL) (R&D systems) as detecting antibody, and recombinant human uPAR (R&D systems) as standard.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Kidney international

Article Title: Expression of urokinase plasminogen activator and its receptor during acute renal allograft rejection.

doi: 10.1046/j.1523-1755.2003.00261.x

Figure Lengend Snippet: Fig. 2. Correlation between urokinase plasminogen activator receptor (uPAR) levels in serum and urine. The urine and serum levels of uPAR show a significant correlation in all groups (R 0.71, P 0.01; Spearman rho test).

Article Snippet: uPAR concentrations in urine and serum were measured by ELISA according to the manufacturer using purified mouse antihuman uPAR monoclonal antibody (mAb) (4 g/mL) (R&D systems, Abingdon, UK) as coating antibody, biotinylated goat-antihuman uPAR antibody (100 ng/mL) (R&D systems) as detecting antibody, and recombinant human uPAR (R&D systems) as standard.

Techniques:

Journal: Kidney international

Article Title: Expression of urokinase plasminogen activator and its receptor during acute renal allograft rejection.

doi: 10.1046/j.1523-1755.2003.00261.x

Figure Lengend Snippet: Fig. 3. Levels of urokinase plasminogen activator receptor (uPAR) in serum (A) and urine (B). Serum and urine levels of uPAR were deter- mined by enzyme-linked immunosorbent assay (ELISA). Results are displayed as mean SEM of the individual serum (A) and urine (B) concentrations of uPAR in transplant patients without rejection, transplant patients with acute rejection type 1, 2, and 3, and healthy subjects. Serum and urine levels of uPAR correspond with severity of rejection. *P 0.05 compared to the nonrejection group; **P 0.05 compared to the control group of healthy volunteers. Both P values were determined by analysis of variance (ANOVA), followed by a Dunnett T3 post hoc test.

Article Snippet: uPAR concentrations in urine and serum were measured by ELISA according to the manufacturer using purified mouse antihuman uPAR monoclonal antibody (mAb) (4 g/mL) (R&D systems, Abingdon, UK) as coating antibody, biotinylated goat-antihuman uPAR antibody (100 ng/mL) (R&D systems) as detecting antibody, and recombinant human uPAR (R&D systems) as standard.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Kidney international

Article Title: Expression of urokinase plasminogen activator and its receptor during acute renal allograft rejection.

doi: 10.1046/j.1523-1755.2003.00261.x

Figure Lengend Snippet: Fig. 4. Immunostaining and in situ hybridization for urokinase plasminogen activator (uPA) and urokinase plasminogen activator receptor (uPAR). Immunostaining (A to F) (red staining with blue hematoxylin counterstaining) and mRNA in situ hybridization (G to L) (blue staining with picro sirius red counterstaining) results for uPA (A, B, C, G, H, and I) and uPAR (D, E, F, J, K, and L) in nonrejected transplants (A, D, G, and J), type I rejection (B, E, H, and K) and vascular rejection (C, F, I, and L). Nonrejected grafts show no expression of uPA/uPAR with immunostaining (A and D). In situ hybridization shows mRNA expression of uPA in a limited number of tubular epithelial cells (G, arrows), and no mRNA expression of uPAR (J) in nonrejected grafts. In type I rejection, the activated lymphocytes show strong positive staining for both uPA and uPAR protein with immunostaining and mRNA using in situ hybridization (B, E, H, and K). Since the overall staining intensity of the in situ hybridization for uPAR was less than for uPA, an inset was placed in (K). Furthermore, the tubular epithelial cells show positive staining for uPA in type I rejection (B and H). In the vascular types of rejection, strong expression of uPA is seen in the entire wall of the involved vessels (C and I), while uPAR is expressed by the endothelium (F and L) [original magnifications 200 and 400 (C, F, I, and L)].

Article Snippet: uPAR concentrations in urine and serum were measured by ELISA according to the manufacturer using purified mouse antihuman uPAR monoclonal antibody (mAb) (4 g/mL) (R&D systems, Abingdon, UK) as coating antibody, biotinylated goat-antihuman uPAR antibody (100 ng/mL) (R&D systems) as detecting antibody, and recombinant human uPAR (R&D systems) as standard.

Techniques: Immunostaining, In Situ Hybridization, Staining, Expressing